| Abstract
| - Enzymes usually cannot withstand the high-temperaturecuring associated with the thick-film fabrication processand require a separate immobilization step in connectionwith the production of single-use biosensors. Wereporton the development of sol−gel-derived enzyme-containingcarbon inks that display compatibility with the screen-printing process. Such coupling of sol−gel andthick-filmtechnologies offers a one-step fabrication of disposableenzyme electrodes, as it obviates the need for thermalcuring. The enzyme-containing sol−gel carbon ink,prepared by dispersing the biocatalyst, along with the graphite powder and a binder, within the sol−gel precursors,is cured very rapidly (10 min) at low temperature (4°C).The influence of the ink preparation conditions is explored, and the sensor performance is evaluated inconnection with the incorporation of glucose oxidase orhorseradish peroxidase. The resulting strips arestablefor at least 3 months. Such sol−gel-derived carboninksshould serve as hosts for other heat-sensitivebiomaterialsin connection with the microfabrication of various thick-film biosensors.
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