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À propos de : Quantification of Biomolecules by ExternalElectrospray Ionization Fourier Transform MassSpectrometry        

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  • Quantification of Biomolecules by ExternalElectrospray Ionization Fourier Transform MassSpectrometry
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  • Fourier transform mass spectrometry (FTMS) is well-known for its capabilities in structural characterizationofmolecules. Recent developments in radio frequencyexcitation, linearized trapping, and accumulation of ionsgenerated from external sources have improved thepotential of FTMS for quantitative analysis. Here, acommercial external electrospray ionization FTMS, employing a linearized ion trap (the Infinity Cell) and anionaccumulation procedure in which ions are deflected off-axis and injected into the trap, is evaluated as ananalyticalmethod for quantifying amino acids, peptides, and proteins. Linear response over ∼2−3 orders ofmagnitudeis observed for singly-charged ions with low coefficientsof variation (generally <10%), and the calibrationcurvesgenerated can be used to quantify structurally similaranalytes with <4% relative error, as shown here forquantification of leucine enkephalin from curves generated by methionine enkephalin. Similar precision isobtained for multiply-charged lysozyme, but over only 1.5orders of magnitude. Some m/z discrimination isobserved as a function of trap accumulation potential for atwo-component cytochrome c/lysozyme mixture.Theresults are promising because they suggest that quantification using liquid chromatography coupled to electrospray FTMS is possible.
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