| Abstract
| - A solid-phase microextraction (SPME) protocol was developed for the quantitative analysis of monocyclic aromatic amines from biological fluids. The headspaceSPME sampling technique was optimized for extractionand concentration of five target analytes (aniline,o-toluidine, 2-chloroaniline, 2,6-dimethylaniline, 2,4,6-trimethylaniline) from urine, blood, and milk. Thetemperature, pH, and ionic strength of the matrix sample weremodified to allow maximum adsorption of the analytesonto the SPME fiber. This method is rapid yetsensitiveand can be completed in 15 min on a 5-mL sample.SPME/GC/MS analysis yielded good reproducibility (RSD>11%) for each analyte from urine, blood, and milk.Method detection limits for the various biologicalfluidswere determined and ranged from 0.40 ppb for 2,4,6-trimethylaniline in urine to 7.7 ppb for aniline in blood.This SPME sampling protocol can be applied to thebiomonitoring of monocyclic aromatic amines from occupational, environmental, and medical exposure.
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