| Abstract
| - A novel chronoamperometric method was developed toestimate the concentration of a neutral ionophore in fixed-site, dioctyl sebacate plasticized, poly(vinyl chloride)-based, ion-selective electrode membranes. The membranes contained between 0.5 and 16 mmol/kg valinomycin. The chronoamperometric technique was used toestimate the valinomycin concentration in freshly prepared membranes and after extraction of some of theionophore from the membranes using dioctyl sebacate.Replicate measurements indicated a relative standarddeviation in the calculated valinomycin concentration ofless than 10%, and these values accurately representedthe true concentration of valinomycin within 10%. Themethod permitted an estimate of the valinomycin concentration after valinomycin was leached from a membrane.The results of preliminary experiments using heparinizeddog blood suggest that blood protein adsorption does notinterfere qualitatively or quantitatively with the analysis.
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