| Abstract
| - It has been demonstrated for the first time that linearrelationships can be obtained between the solid-matrixphosphorescence (SMP) and the percent modification ofbenzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE)−DNA adducts. The samples of DNA were modified withBPDE at levels of 5.0 × 10-3, 1.0 × 10-3, 5.0 × 10-4,1.0 × 10-4, 5.0 × 10-5, and 1.0 × 10-5%. In addition,the changes in the SMP intensities of a given percentageof DNA adduct were investigated as a function of samplesize, and linear relationships were also acquired. With thedifferent percentages of modified DNA, very good reproducibility of the SMP signals was obtained. Data wereacquired with both Whatman 1PS paper and 30% TlNO3/sodium acetate as solid matrixes. The limit of detectionfor the BPDE−DNA adducts was 2 adducts in 107 basesfor both Whatman 1PS paper and 30% TlNO3/sodiumacetate. In addition, it was shown that it would beimportant to develop a standard procedure for the preparation of the BPDE−DNA samples if different batches ofDNA were used in the preparation procedure.
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