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| - Amperometric Detection of Histamine with aMethylamine Dehydrogenase Polypyrrole-BasedSensor
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| - Methylamine dehydrogenase (MADH) has been immobilized in a polypyrrole (PPy) film on an electrode surfaceand used as an amine sensor for the determination ofprimary amines. Its response to histamine has beencharacterized in detail. The PPy film containing MADHwas formed electrochemically on a gold minielectrode (1-mm diameter) in the presence of ferricyanide. The filmwas then coated with Nafion. This enzyme electrode didnot require any additional cofactors and was not sensitiveto oxygen. It exhibited a maximum response current tohistamine at applied potentials of 0.24−0.33 V and at pH7.5−8.5. This MADH−PPy sensor exhibited a responsetime of less than 3 s. The immobilized MADH on theelectrode exhibited Michaelis−Menten behavior similarto that of the free enzyme in solution with a Km value of1.3 mM. This sensor could be used to reliably detecthistamine over a concentration range from approximately25 μM to 4 mM. This is the first example of a biosensorthat uses an immobilized enzyme that possesses thetryptophan tryptophylquinone prosthetic group.
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