| Abstract
| - A novel detection system using both emission energytransfer and time-resolved fluorometry (TRF) was developed, with a europium chelate as the energy donor and anovel fluorophore SNR1, excitable with long-wavelengthlight corresponding to europium emission, as the energyacceptor. When the donor and acceptor molecules weremixed in solution, energy transfer was observed withoutdirect attachment of the donor and the acceptor, via adiffusion-enhanced energy-transfer mechanism. Thus, theacceptor emission can be detected as a long-lifetimefluorescence in TRF. When the fluorescence propertiesof the acceptor molecule are changed by interaction withan enzyme or other bioactive molecule, the change canbe detected as a long-lived sensitized emission. If wedevelop or select suitable acceptor molecules, this simpleand convenient system should be applicable to a widevariety of bioactive molecules. Since it is based on TRF,it can be used for high-resolution assay.
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