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| - Analysis of Human Skin Emanations by GasChromatography/Mass Spectrometry. 2.Identification of Volatile Compounds That AreCandidate Attractants for the Yellow FeverMosquito (Aedes aegypti)
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| - Volatile compounds emanated from human skin werestudied by gas chromatography/mass spectrometry (GC/MS). The purpose of this study was to identify compoundsthat may be human-produced kairomones which are usedfor host location by the mosquito, Aedes aegypti (L.). Theprocedure used to collect volatiles was chosen becauseof prior knowledge that attractive substances can betransferred from skin to glass by handling. Laboratorybioassays have shown that the residuum on the glassremains attractive to mosquitoes until the compounds ofimportance evaporate. The sampling and analytical procedures modeled the above-cited process as closely aspossible except that the evaporation of compounds fromthe glass surface was accomplished by thermal desorptionfrom glass beads in a heated GC injection port. This madepossible the solventless injection of volatiles onto thecolumn. The compounds were cryofocused on the headof the column with liquid nitrogen prior to GC separation.A single stage of mass spectrometry on a triple quadrupoleinstrument was used for mass analysis. A combination ofelectron ionization and pulsed positive ion/negative ionchemical ionization modes on two different GC columns(one polar, one relatively nonpolar) was used to identifymost of the 346 compound peaks detected by thistechnique.
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