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| - Multimicrobial Sensor Using MicrostructuredThree-Dimensional Electrodes Based on SiliconTechnology
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| - Two microbial strains with different substrate spectra wereimmobilized separately within a single biosensor chipfeaturing four individually addressable platinum electrodes. These were sputtered onto the inner surface offour isolated pyramidal cavities (“containments”) micromachined on a silicon wafer. The biosensor chip wasintegrated into a flow-through system to measure theoxygen consumption of the immobilized microorganismsin the presence of assimilable analytes. As a modelsystem, a yeast for the determination of biochemicaloxygen demand (BOD) and a strain capable of degradingpolycyclic aromatic hydrocarbons (PAH) were chosen. Itwas shown that the simple and mass-producible containment sensor exhibits good performance data: lowerdetection limit 0.1 mg/L naphthalene and 1 mg/L sensor-BOD; calibration range up to 30 mg/L; precision 3−6%;response time 2−3 min; service life up to 40 days; shelflife at 4 °C 6 months. The versatility of the multimicrobialsensor was demonstrated by measuring ordinary municipal wastewater samples as well as various aqueoussamples contaminated with PAH. The concept of a multimicrobial sensor not only enlarges the substrate spectrum for sum parameters such as BOD but leads toadditional information which allows for a more differentiated and immediate knowledge of sample composition.Using chemometrical data analysis, the multimicrobialsensor lays a foundation for developing an “electronictongue”.
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