| Abstract
| - In both atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) and vacuum MALDI, thelaser typically illuminates the analyte on the front side ofan opaque surface (reflection geometry). Another configuration consisting of laser illumination through the samplebackside (transmission geometry) has been used inconventional MALDI; however, its use and the numberof reports in the literature are limited. The viability oftransmission geometry with AP MALDI is demonstratedhere. Such a geometry is simple to implement, eliminatesthe restriction for a metallic sample holder, and allowsfor the potential analysis of samples on their nativetransparent surfaces, e.g., cells or tissue sections onslides.
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