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| - Characterization and Quantitative Analysis withGC/TOFMS Comparing Enhanced Separation withTandem-Column Stop-Flow GC and SpectralDeconvolution of Overlapping Peaks
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| - Time-of-flight mass spectrometry is unique in that ionabundance ratios are constant over the chromatographicpeak profile provided that the peak contains only onecomponent. This provides the means for the automatedfinding and spectral deconvolution of overlapping chromatographic peaks from completely unknown mixturesif the mass spectra for the overlapping components aresufficiently unique. This can greatly reduce the chromatographic resolution requirements, which allows for veryrapid quantitative analysis as well as for high-speedmixture characterization. High-speed GC with stop-flowoperation of a series-coupled column ensemble can beused to completely separate some component pairs thatcoelute from the column ensemble, thus eliminating theneed for spectral deconvolution of those mixture components. This provides two options for high-speed qualitativeand quantitative analysis, using either the mass spectrafrom deconvoluted overlapping peaks or the mass spectrafrom the completely separated peaks obtained with stop-flow operation of the tandem column ensemble. Theseoptions are compared with respect to the similarity forspectral matching with a library and to peak area linearitywith concentration, calibration plot correlation coefficients, and shot-to-shot reproducibility.
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