Abstract
| - A novel surface enzymatic amplification method thatutilizes RNA microarrays in conjunction with the enzymeRNase H is developed for the ultrasensitve detection andanalysis of target DNA molecules. The enzyme RNase His shown to selectively and repeatedly destroy RNA fromRNA−DNA heteroduplexes on gold surfaces; when usedin conjunction with the label-free technique of surfaceplasmon resonance imaging, multiple DNA targets can bedetected at a concentration of 10 fM on a single chip. Inaddition, this method is utilized for the sequence-specificdetection of the TSPY gene in both purified and unpurifiedPCR products. Finally, in a series of kinetics measurements, the initial rate of hydrolysis is shown to dependdirectly on the surface concentration of DNA−RNA heteroduplexes.
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