| Abstract
| - Pressurized capillary electrochromatography (pCEC) wascoupled with electrospray ionization mass spectrometry(ESI-MS) using a coaxial sheath liquid interface. It wasused for separation and analysis of peptides and proteins.The effects of organic modifier and applied voltage onseparation were investigated, and the effects of pH valueof the mobile phase and the concentration of the electrolyte on ESI-MS signal were investigated. The resolutionand detection sensitivity with different separation methods(pCEC, capillary high-performance liquid chromatography) coupled on-line with mass spectrometry were compared for the separation of a peptide mixture. To evaluatethe feasibility and reliability of the experimental setup ofthe system, tryptic digests of cytochrome c and modifiedprotein as real samples were analyzed by using pCEC-ESI-MS.
|
