Documentation scienceplus.abes.fr version Bêta

À propos de : Label-Free Reading of Microarray-BasedImmunoassays with Surface Plasmon ResonanceImaging        

AttributsValeurs
type
Is Part Of
Subject
Title
  • Label-Free Reading of Microarray-BasedImmunoassays with Surface Plasmon ResonanceImaging
has manifestation of work
related by
Author
Abstract
  • A simple method is presented for patterning of proteinantigens at a gold surface for use in surface plasmonresonance (SPR) imaging experiments. Microfluidic devices fabricated from poly(dimethylsiloxane) were usedto flow various fluids over a gold substrate in spatiallydefined channels. This technique was used to pattern thesurface chemistry of the gold as well as to adsorb antigensfrom solution to the modified substrates. The resultingantigen arrays were probed with complementary antibodies in order to demonstrate the effectiveness of thepatterning for antibody capture experiments. SPR imagingwas used to aid in the optimization of array fabricationand to observe the interactions of unlabeled antibodieswith these microarrays. This work presents a means offabricating microarrays with controlled surface density ofantigens. SPR imaging provides both quantitative andqualitative evaluation of antibody binding in a label freeformat.
article type
is part of this journal



Alternative Linked Data Documents: ODE     Content Formats:       RDF       ODATA       Microdata