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| - Magnetic and Gold-Coated Magnetic Nanoparticlesas a DNA Sensor
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| - In this study, we report the chemical synthesis andfunctionalization of magnetic and gold-coated magneticnanoparticles and the immobilization of single-strandedbiotinylated oligonucleotides onto these particles. Selectedsequences specific to the BRCA1 gene were used as a testplatform. The binding of oligonucleotides to these particleswas achieved through a streptavidin−biotin bridge via acarbodiimide activation protocol. Particle size and oligonucleotide attachment were confirmed by transmissionelectron microscopy; oligonucleotide binding was characterized by Fourier transform infrared spectroscopy andhybridization confirmed by fluorescence emission fromthe fluorophore attached to the target oligonucleotidestrand. The rate of hybridization was measured using aspectrofluorometer and a microarray scanner. The rateof hybridization of oligonucleotides bound to the synthesized particles depends on the inorganic support materialand its surface chemistry. The rate of hybridizationincreased concomitantly with the concentration of theprobe and the target in the reaction medium. Furthermore, exposure of probe and target oligonucleotide to acombination of target and noncomplementary DNA strandreduced the rate of hybridization, possibly because ofsteric crowding in the reaction medium and cross-linkingbetween reacting oligonucleotides and the noncomplementary strands. The study undertaken opens severalpossibilities in bioconjugate attachment to functionalizediron and iron nanocomposite structures for controlledmanipulation and handling using magnetic fields.
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