| Abstract
| - The development of a new ion mobility/mass spectrometryinstrument that incorporates a multifield drift tube/ionfunnel design is described. In this instrument, individualcomponents from a mixture of ions can be resolved andselected on the basis of mobility differences prior tocollisional activation inside the drift tube. The fragmentions that are produced can be dispersed again in a secondion mobility spectrometry (IMS) region prior to additionalcollisional activation and MS analysis. The result is anIMS−IMS analogue of MS−MS. Here, we describe thepreliminary instrumental design and experimental approach. We illustrate the approach by examining thehighly characterized bradykinin and ubiquitin systems.Mobility-resolved fragment ions of bradykinin show thatb-type ions are readily discernible fragments, because theyexist as two easily resolvable structural types. Currentlimitations and future directions are briefly discussed.
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