| Abstract
| - A novel approach for carotenoid analysis has been developed. Orange essential oil and juice carotenoids wereseparated by means of comprehensive dual-gradient elution HPLC, using normal phase with a microbore silicacolumn in the first dimension (first D), reversed phasewith a monolithic C18 column in the second dimension(second D), and a 10-port switching valve as an interface.An on-line photodiode array detector was used in orderto obtain absorption spectra. Peak identification wasobtained by combining retention data with the UV−visiblespectra.
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