| Abstract
| - A parallel nanoliter microfluidic analysis system based oncapillary action, centrifugal force, and hydrophobic barriers is described. The precision of 112 parallel volumedefinition operations is determined to 0.75% CV at 200nL using the individual sample introduction structure. For20 nL, the actual measurement error is the dominatingfactor, with a combined error of 1.9% CV. Individualdispensing as well as dispensing through a commondistribution channel is described. The volume definitionprecision for the common distribution channel is 1.6%CV for 200 nL. Unlike the dominating forces in microliter-sized channel systems, we describe hysteresis effects asexerting a major influence, which needs to be consideredin order to control the operation and design of discretenanoliter fluidics. Hydrophobic patches at the corners ofthe rectangular channel control corner-enhanced wicking.Excellent flow control of 1 and 2 nL/s is achieved usinga predefined spin program.
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