Documentation scienceplus.abes.fr version Bêta

À propos de : Hairpin-DNA Probe for Enzyme-AmplifiedElectrochemical Detection of Legionellapneumophila        

AttributsValeurs
type
Is Part Of
Subject
Title
  • Hairpin-DNA Probe for Enzyme-AmplifiedElectrochemical Detection of Legionellapneumophila
has manifestation of work
related by
Author
Abstract
  • An electrochemical genosensor for the detection of nucleicacid sequences specific of Legionella pneumophila isreported. An immobilized thiolated hairpin probe iscombined with a sandwich-type hybridization assay, usingbiotin as a tracer in the signaling probe, and streptavidin-alkaline phosphatase as reporter molecule. The activityof the immobilized enzyme was voltammetrically determined by measuring the amount of 1-naphthol generatedafter 2 min of enzymatic dephosphorylation of 1-naphthylphosphate. The sensor allows discrimination between L.pneumophila and L. longbeachae with high sensitivityunder identical assay conditions (no changes in stringency). A limit of detection of 340 pM L. pneumophilaDNA, and a linear relationship between the analyticalsignal and the logarithm of the target concentration to 2μM were obtained. Experimental results show the superior sensitivity and selectivity of the hairpin-based assaywhen compared with analogous sandwich-type assaysusing linear capture probes.
article type
is part of this journal



Alternative Linked Data Documents: ODE     Content Formats:       RDF       ODATA       Microdata