Documentation scienceplus.abes.fr version Bêta

À propos de : Preparation and Purification of an End to End CoupledmEGF−Dextran Conjugate        

AttributsValeurs
type
Is Part Of
Subject
Title
  • Preparation and Purification of an End to End CoupledmEGF−Dextran Conjugate
has manifestation of work
related by
Author
Abstract
  • The amino terminus of mouse epidermal growth factor (mEGF) was coupleddirectly to the aldehydeend of dextran through a reductive amination procedure. Thehighest coupling efficiency was ∼80%and could be reached after ∼24 h of reaction time at pH 8. Gelfiltration on Sephadex G-50 Fineremoved free mEGF from the conjugate. Preparative polyacrylamidegel electrophoresis was used toseparate the conjugate from excess noncharged dextran. Theconjugate bound specifically to the EGFreceptor on cultured glioma cells as shown in displacement tests withfree mEGF. The conjugatewas stable in the pH interval 4−9, in 2 M sodium chloride, in 7 Murea, and in human serum andcould still bind to the EGF receptor after such treatments. Theconjugates are candidates for targetednuclide therapy.
article type
is part of this journal



Alternative Linked Data Documents: ODE     Content Formats:       RDF       ODATA       Microdata