Toward an in Vitro Test for the Diagnosis of Allergy to Penicillins.Synthesis, Characterization, and Use of β-Lactam and β-LactamMetabolite Poly-l-lysines Which Recognize Human IgE Antibodies
Conjugates of poly-l-lysine (PLL) containing a penicillin or a penicilloyl residue were prepared andcharacterized by 1H NMR and by size-exclusion (SE) HPLC. These conjugates were used in a radioallergo sorbent tests (RAST) test for the determination of allergy toward β-lactams. The chemiometricevaluation of the data indicates that allergy to amoxycillin is different from allergy to the otherβ-lactams tested. Furthermore, careful chemical characterization of the conjugates appears to be crucialto obtain meaningful information from the RAST data.
