| Abstract
| - New, highly amino-substituted dextran or aminodextran (hereafter denoted Amdex) of various sizesbetween about 20 and 1000 kDa molecular mass and degrees of amino-substitution between 7 and40% were prepared and characterized by elemental analyses and polyacrylamide gel electrophoresis.These aminodextrans together with others commercially available were shown by static light scattering,viscosity, and refractive index measurements to adopt a globular structure in aqueous salt solutions.Antibody and fluorescent protein dye, phycoerythrin, or its tandems with cyanin 5.1 and TEXAS RED,were covalently conjugated to the aminodextrans. The conjugates contained multiple dye moleculesand were shown by dynamic light scattering and scanning electron microscopy to assume eitherglobular structure or aggregates thereof. Streptavidin could be substituted for antibody to preparestreptavidin−aminodextran−PE conjugates, which were then used with biotinylated antibody to labelsubpopulations of white blood cells. The conjugates yielded up to 20-fold amplification of fluorescenceintensity over direct antibody−dye conjugates in labeling white blood cells for flow cytometry.
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