| Abstract
| - The synthesis of benzylpenicilloyl-containing dendrimers has been achieved by a convenient procedureinvolving quantitative functionalization of the terminal amino groups of the three Starbust PAMAMgenerations used (Gn; n = 0, 1, 2). All these densely penicilloylated dendrimers (GnP) exhibit similar,simple NMR spectroscopic data suggesting highly symmetric structures and a monodisperse nature,and the results obtained from MALDI-TOF-MS demonstrate their exact chemical composition. Theuse of PAMAM dendrimers has allowed us to synthesize, for the first time, carrier benzylpenicilloylconjugates (GnP) of precisely defined chemical structure. The attempts to synthesize G2P show thatforced experimental conditions are not always useful for the functionalization of the dendrimer,especially in introducing bulky groups. The initial results with sera from patients with different RASTlevels were positive and thus suggestive that inhibition occurs, so recognition exists; we can thereforeconclude that the hapten−carrier (dendrimer) conjugates studied mimic recognition with naturalhapten−carrier (protein) conjugates.
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