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| - Template Strand Gap Bypass Is a General Property of Prokaryotic RNAPolymerases: Implications for Elongation Mechanisms
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| - It has previously been shown that T7 RNA polymerase is capable ofbypassing gaps on thetemplate strand ranging in size from 1 to 24 nucleotides. This aswell as other observations suggested arole for the nontemplate strand during elongation. To establishthe generality of this gap bypassing event,we have extended these studies to SP6 and Escherichia coliRNA polymerases. SP6 RNA polymerasebypasses template gaps from 1 to 19 nucleotides in size with variousdegrees of efficiency and producesrunoff transcripts of decreasing length corresponding to increasing gapsize. RNA sequence analysis ofthe resulting runoff transcripts revealed that SP6 RNA polymerasefaithfully transcribed both parts of thetemplate strand flanking the gapped region. Similar experimentswere carried out with E. coli RNApolymerase (a multiple subunit enzyme) and indicate that it is alsocapable of gap bypass albeit withreduced efficiency compared to T7 and SP6 RNA polymerases. Itappears that the ability to bypass gapspresent on the DNA template strand is a general property of prokaryoticRNA polymerases. These resultshave implications with respect to the mechanism of elongation and therole of the nontemplate strand intranscription.
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