| Abstract
| - A cDNA encoding mouse pseudouridine synthase 3 (mPus3p) has been cloned. The predictedprotein has 34% identity with yeast pseudouridine synthase 3 (Pus3), an enzyme known to formpseudouridine at positions 38 and 39 in yeast tRNA. The cDNA is 1.7 kb, and when used as a probe ona Northern blot of total RNA from mouse tissues or cells in culture, a band at 1.8 kb was observed. Theopen reading frame codes for a protein of 481 amino acids with a predicted molecular mass of 55 552Da. When mPus3p was in vitro translated and used in reactions with tRNA substrates from both yeastand humans, uridines at position 39 were modified to pseudouridine. In a tRNA substrate with a uridineat position 38 (human tRNALeu), there was very slight formation of pseudouridine at that position afterincubation with mPus3p.
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