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| - Polar Group Burial Contributes More to Protein Stability than Nonpolar GroupBurial
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| - On the basis of studies of Asn to Ala mutants, the gain in stability from burying amide groupsthat are hydrogen bonded to peptide groups is 80 cal/(mol Å3). On the basis of similar studies of Leu toAla and Ile to Val mutants, the gain in stability from burying −CH2− groups is 50 cal/(mol Å3). Thus,the burial of an amide group contributes more to protein stability than the burial of an equivalent volumeof −CH2− groups. Applying these results to folded proteins leads to the surprising conclusion that peptidegroup burial makes a larger contribution to protein stability than nonpolar side chain burial. Several studieshave shown that the desolvation penalty for burying peptide groups is considerably smaller than generallythought. This suggests that the hydrogen bonding and van der Waals interactions of peptide groups in thetightly packed interior of folded protein are more favorable than similar interactions with water in theunfolded protein.
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