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| - Biocompatible Sol−Gel Route for Encapsulation ofLiving Bacteria in Organically Modified Silica Matrixes
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| - An alcohol-free sol−gel route based on the controlled vacuum evaporation of the alcoholthat results as a byproduct of the hydrolysis and condensation of alkoxyde precursors isdescribed for the encapsulation of living bacteria. The ability of the encapsulated cells (agenetically engineered E. coli strain) to express a fluorescent protein in response to thepresence of dicyclopropyl ketone has been used to study both the biocompatibility of theencapsulation route and the viability of the encapsulated cells with aging time. Thus, up to95% of the integrity of the living cell along the encapsulation process has been preservedthrough alcohol removal of the starting sol. In addition, the use of organically modified ratherthan pure silica gels as the host matrix has shown a notable improvement in the viabilityof encapsulated cells (18 versus 6 days, respectively).
- The current work presents an alcohol-free sol−gel route beginning from organically modified precursors for the encapsulation of living cells. The ability of the encapsulated cells (a genetically engineered E. coli strain) to express a fluorescent protein in response to the presence of dicyclopropyl ketone has been used to study both the biocompatibility of the encapsulation route and the viability of the encapsulated cells with aging time.
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