| Abstract
| - A set of fluorescent sensors was used to investigate the selective labeling of bone microdamage. It was shown that selective labeling occured for all sensors used, most likely due to binding at free lattice sites. Graphic: Transverse section of bone labeled with calcium orange showing an osteon with cell lacunae, canaliculi, and a central Haversian canal.
- This paper describes the characterization of microdamage in bones histologically, spectroscopically,and by surface analysis. A set of fluorescent (photoinduced electron transfer, PET) sensors, bearingphenyliminodiacetate moieties as ion receptors, was used to investigate the selective labeling ofmicrodamage in bones, which can have significant value for analysis of bone structures in humans.Scratched and unscratched surfaces of the bone were studied using fluorescence microscopy, Ramanspectroscopy, SEM, and EDXA. These results were compared to those using Rose Bengal dye. Theoverall results show that selective labeling of scratches occurred for all the fluorescent sensors, whichcan be attributed to the interaction of dyes via binding at free lattice sites (via the receptors), throughionic interactions with free lattice sites or by incorporation in the broken lattices. The principal mode ofoperation is most likely due to the binding of these sensors to Ca(II) at microdamage sites.
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