| Abstract
| - UV irradiation of oligodeoxynucleotides at 254 nm generates several different types of DNAphotoproducts, such as cis-syn cyclobutane pyrimidine dimers, pyrimidine[6−4]pyrimidonephotoproducts and their Dewar valence isomers, and thymine-adenine photoproducts (TA*).Nuclease P1 degrades the oligodeoxynucleotide photoproducts to small photoproduct-containingtrinucleotides which are more amenable to tandem mass spectrometry (MS/MS) and HPLC.Product-ion mass spectra of these digestion products give characteristic fragmentations,allowing us to identify quickly the types of photomodifications. The results also show thatmass spectrometry will be a tool for studying enzyme reaction mechanisms because it candetermine rapidly and with high sensitivity the structures of the products that are generated.
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