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| - Bacterial Bioassay for Rapid andAccurate Analysis of Arsenic inHighly Variable GroundwaterSamples
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| - In this study, we report the first ever large-scaleenvironmental validation of a microbial reporter-basedtest to measure arsenic concentrations in natural waterresources. A bioluminescence-producing arsenic-induciblebacterium based on Escherichia coli was used as thereporter organism. Specific protocols were developed withthe goal to avoid the negative influence of iron ingroundwater on arsenic availability to the bioreportercells. A total of 194 groundwater samples were collectedin the Red River and Mekong River Delta regions ofVietnam and were analyzed both by atomic absorptionspectroscopy (AAS) and by the arsenic bioreporter protocol.The bacterial cells performed well at and above arsenicconcentrations in groundwater of 7 μg/L, with an almostlinearly proportional increase of the bioluminescence signalbetween 10 and 100 μg As/L (r2 = 0.997). Comparisonsbetween AAS and arsenic bioreporter determinations gavean overall average of 8.0% false negative and 2.4% falsepositive identifications for the bioreporter prediction at theWHO recommended acceptable arsenic concentration of10 μg/L, which is far better than the performance of chemicalfield test kits. Because of the ease of the measurementprotocol and the low application cost, the microbiologicalarsenic test has a great potential in large screeningcampaigns in Asia and in other areas suffering from arsenicpollution in groundwater resources.
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