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| - Selective Biocatalysis in Bacteria Controlled by Active MembraneTransport
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| - Membrane reactors are attractive when retention of biocatalysts is desirable and when chemicalconversion or selectivity can be enhanced by selective permeability of reactants. Unlike polymeror inorganic membranes, biological membranes have transport proteins that use cellular energyto selectively pump components against concentration gradients. This paper analyzes thetransport of polynuclear aromatic hydrocarbons (PAHs) across the cell membranes of Pseudomonas fluorescens, an organism that oxidizes aromatic compounds. Experimental data for PAHswere consistent with a model for uptake and energy-dependent transport controlled by thepermeability of the outer cell membrane permeability. A model for the enzymatic reaction ofmixtures of PAHs showed that energy-dependent transport out of the cell increases the selectivityfor the reaction of the less permeable component. Consequently, selective transport may be auseful tool in engineering the metabolism of microorganisms, especially for less hydrophobiccompounds with lower permeation rates across biological membranes.
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