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| - Methods for Measurement of Intermolecular NOEs byMultinuclear NMR Spectroscopy: Application to aBacteriophage λ N-Peptide/boxB RNA Complex
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| - New pulse schemes for recording intermolecular NOEs in a molecularcomplex consisting of 15N,13Clabeled and unlabeled components are presented. The pulsesequences select for magnetization transferred fromprotons on the unlabeled component to proximal protons of the labeledmolecule. Filtering (suppression of signalfrom 13C labeled molecules) is accomplished using adiabatic13C inversion pulses which are swept at a ratewhichis tuned according to the one-bond 1H−13Cscalar coupling vs carbon chemical shift profile of the labeledmoleculein the complex. Significantly improved spectra are obtainedrelative to data recorded with other purging schemes.Improvements are demonstrated in experiments where intermolecularNOEs between labeled RNA-unlabeled peptideand labeled protein−unlabeled peptide are recorded. A discussionof structural information obtained for a complexof the amino-terminal arginine rich domain of the N protein frombacteriophage λ and boxB RNA using the newmethodology is presented.
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