This communication describes a new molecular recognition chip using a semi-wet microenvironment provided by a self-assembled hydrogel. On the basis of the evidence that the molecular recognition capability of artificial chemosensors are practically retained even in the hydrogel compared to those in aqueous solution, we miniaturized the functionalized hydrogel to produce an unprecedented molecular recognition chip. We believe that the present noncovalent immobilization method is generally applicable to many chemosensors, which leads to a unique semi-wet sensor chip suitable to convenient and high-throughput assay to plural analytes.