| Abstract
| - At elevated levels, homocysteine (Hcy, 1) is a risk factor for cardiovascular diseases, Alzheimer'sdisease, neural tube defects, and osteoporosis. Both 1 and cysteine (Cys, 3) are linked to neurotoxicity.The biochemical mechanisms by which 1 and 3 are involved in disease states are relatively unclear. Herein,we describe simple methods for detecting either Hcy or Cys in the visible spectral region with the highestselectivity reported to date without using biochemical techniques or preparative separations. Simple methodsand readily available reagents allow for the detection of Cys and Hcy in the range of their physiologicallyrelevant levels. New HPLC postcolumn detection methods for biological thiols are reported. The potentialbiomedical relevance of the chemical mechanisms involved in the detection of 1 is described.
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