| Abstract
| - A simple and rapid on-line analytical system was configured for theevaluation of the photostabilityof various food coloring agents constantly exposed to light. Thescreening test for photostability offood coloring agents was carried out in the system consisting of 60 cmof Teflon tubing for ultraviolet(UV) irradiation with a flow rate of mobile phase (water/acetonitrile= 1:1, v/v) at 1 mL/min. Whenthe products derived from photochemical reaction of phloxine (R-104),which is recognized to exhibitchemiluminescence induced by UV irradiation, were separated, severalpeaks were confirmed onthe chromatogram in this system. Furthermore, the peak ofchemilumigenics observed on thechromatogram of photon-counting chemiluminescence detection correspondsto the new peaks onthe UV chromatogram. Also, the presence of phenoxy radical(g = 2.0061) formed by UV irradiationof R-104 was confirmed with an electron spin resonance spectrometer,indicating a reasonablerelationship (r = 0.9041) between the formation of freeradical and the chemiluminescencephenomenon. Keywords: Photostability; phloxine; chemiluminescence; on-line system; ESR;free radical formation
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