| Abstract
| - Sabadilla, an insecticide widely used by organic farmers, can beseparated into five of its components(veratridine, cevadine, cevine, cevacine, and sabadine) byhigh-performance liquid chromatography(HPLC) and detected by atmospheric pressure chemical ionization liquidchromatography massspectrometry (APCI/LCMS). Using APCI/LCMS in the selected ionmonitoring mode as thedeterminative step, an analytical method has been developed for thedetection and quantitation ofthe two largest sabadilla components, veratridine and cevadine, inlettuce and cucumbers spikedwith veratrine. The insecticides are extracted withacetonitrile/water, and the extract is cleanedup by solid phase extraction (SPE) on a C-18 cartridge. The limitsof detection (calculated at asignal to noise ratio of 10 to 1) of veratridine and cevadine incucumbers and lettuce are 1−2 ppb,and the recoveries range from 74% to 101%. Keywords: Organic farming; sabadilla; veratridine; cevadine; cevine;cevacine; sabadine; high-performance liquid chromatography (HPLC); atmospheric pressure chemicalionization liquidchromatography mass spectrometry (APCI/LCMS)
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