| Abstract
| - The methanolic extract of saffron was prefractionated with the aid ofmultilayer coil countercurrentchromatography (MLCCC). After subsequent purification of certainfractions by high-performanceliquid chromatography, the following glycoconjugates were isolated andidentified on the basis oftheir spectral (UV, MS, NMR) data: the β-d-glucosidesof (4R)-4-hydroxy-3,5,5-trimethylcyclohex-2-enone (5),(4S)-4-hydroxy-3,5,5-trimethylcyclohex-2-enone(6), and(4S)-4-(hydroxymethyl)-3,5,5-trimethylcyclohex-2-enone (7), as well as theβ-d-gentiobiosyl ester of2-methyl-6-oxohepta-2,4-dienoicacid (4). In addition to these new natural products,known saffron constituents, i.e., the di-β-d-gentiobiosyl andβ-d-gentiobiosyl-β-d-glucopyranosyl estersof crocetin, have also been isolated andseparated using MLCCC. Keywords: (4R)-4-Hydroxy-3,5,5-trimethylcyclohex-2-enone4-O-β-d-glucopyranoside;(4S)-4-hydroxy-3,5,5-trimethylcyclohex-2-enone4-O-β-d-glucopyranoside;(4S)-4-(hydroxymethyl)-3,5,5-trimethylcyclohex-2-enone 4-O-β-d-glucopyranoside;β-d-gentiobiosyl ester of2-methyl-6-oxohepta-2,4-dienoic acid; crocetin derivatives; carotenoid degradation products;multilayer coil countercurrentchromatography; saffron; Crocus sativus;Iridaceae
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