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À propos de : Determination of Random- and Blockwise-type De-esterified Pectinsby Capillary Zone Electrophoresis        

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  • Determination of Random- and Blockwise-type De-esterified Pectinsby Capillary Zone Electrophoresis
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  • Capillary zone electrophoresis (CZE) was employed to determine the correlations between migrationtime and degree of esterification (DE) of pectinesterase-de-esterified pectins (PDPs) and alkaline-de-esterified pectins (ADPs) using 50 mM phosphate buffer (pH 6.5) as carrier electrolyte solutionand 15 KV as applied voltage. Results showed that pectins with higher DEs exhibited shortermigration times. Linear correlation (r = 0.995) between migration time and DE of ADPs wasobserved, whereas down-curve correlation in PDPs was observed, regardless of the capillary lengthused (effective length, 30 and 60 cm). In addition, PDP appeared to migrate faster than ADP withthe same DE under the same experimental conditions. Keywords: Degree of esterification; random-type de-esterified pectin; blockwise-type de-esterifiedpectin; capillary zone electrophoresis
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