| Attributs | Valeurs |
|---|
| type
| |
| Is Part Of
| |
| Subject
| |
| Title
| - Effect of Caffeic Acid Phenethyl Ester, an Antioxidant fromPropolis, on Inducing Apoptosis in Human Leukemic HL-60 Cells
|
| has manifestation of work
| |
| related by
| |
| Author
| |
| Abstract
| - Caffeic acid phenethyl ester (CAPE) is an active component isolated from propolis. The aim of thisstudy was to investigate the mechanism of CAPE-induced apoptosis in human leukemic HL-60 cells.It was found that CAPE entered HL-60 cells very quickly and then inhibited their survival in aconcentration- and time-dependent manner. CAPE induced characteristic DNA fragmentation andmorphological changes typical of apoptosis in these cells. Estimation of the apoptotic percentageshowed a time-dependent increase after CAPE (6 μg/mL) treatment (up to 66.7 ± 2.0% at 72 h).Treatment with CAPE caused rapid activation of caspase-3 after 4 h, down-regulation of Bcl-2expression after 6 h, and up-regulation of Bax expression after 16 h. These results suggest thatCAPE is a potent apoptosis-inducing agent; its action is accompanied by activation of caspase-3,down-regulation of Bcl-2, and up-regulation of Bax in human leukemic HL-60 cells. Keywords: Caffeic acid phenethyl ester (CAPE); apoptosis; caspase-3; Bcl-2; Bax
|
| article type
| |
| is part of this journal
| |
