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| - Inactive Corrinoid-Compound Significantly Decreases in Spirulinaplatensis Grown in a Cobalt-Deficient Medium
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| - Spirulina platensis NIES-39 was grown under open culture system in the presence or absence ofCoSO4 (12 μg/L) and/or vitamin B12 (10 μg/L) to confirm whether CoSO4 and/or vitamin B12 stimulateor are essential for growth of the algal cells and for accumulation of vitamin B12. The addition ofCoSO4 and/or vitamin B12 could not affect both cell growth and cell yield of the alga. The amountof corrinoid-compound was increased significantly by the addition of CoSO4 but not by vitamin B12.A C18 reversed-phase HPLC pattern of the Spirulina corrinoid-compound increased by the additionof CoSO4 was identical to that of authentic pseudovitamin B12, which is inactive for human. Theseresults indicate that the algal cells grown in the absence of CoSO4 are suitable for use of humanhealth foods because the inactive corrinoid-compound can be reduced significantly. Keywords: Vitamin B12; pseudovitamin B12; algal health food; spirulina tablet; intrinsic factor
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