| Abstract
| - Six hens were intramuscularly (im) immunized once a week for 3 weeks using chicken egg whitelysozyme (LS) as antigen. Antibody (immunoglobulin in yolk, IgY) ELISA values of 103-fold dilutedyolk were almost as high as 1.879 in the sixth week and maintained a value of 0.756 in the eighthweek after the initial immunization treatment. The purification efficiency (specific activity of purifiedIgY against LS/specific activity of antibody in yolk against LS) of IgY specific against LS isolated bylaboratory-prepared LS-bound (IgY-) Sepharose 4 Fast Flow immunoaffinity column was ∼3380. Byapplying various amounts (0−22 mg) of the thusly obtained IgY specific against LS to theimmunoaffinity column, the binding capacity (qm) and dissociation constant (Kd, M-1) of suchimmunoaffinity gel for IgY against LS were found to be 0.68 mg of IgY/mL of wet gel (0.54 mg ofIgY/mg of LS) and 7.13 × 10-6 M, respectively, as determined by Langmuir-type adsorption isotherms. Keywords: Lysozyme; immunoglobulin in yolk; immunoaffinity chromatography; binding capacity;dissociation constant
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