| Abstract
| - Bisphenol A was coupled, after derivatization into a suitable hapten, to bovine serum albumin andovalbumin in order to produce immunizing and coating antigens. The immunizing antigens wereinjected into chickens, which allowed the isolation of specific bisphenol A immunoglobulins from theegg yolk. These antibodies were used in an indirect competitive enzyme-linked immunosorbent assayfor the determination of bisphenol A in aqueous solutions. Various parameters, influencing the assaysensitivity, were evaluated. The applicability of the assay for the determination of bisphenol A in milkwas also studied. The assay was not as sensitive as other analytical techniques used in bisphenolA analysis, since typical I50 levels of 2.5 μM were reached in aqueous solutions. This studynevertheless illustrates the usefulness and the potency of chicken antibodies in the analysis ofmigration residues from packaging materials using immunochemical techniques. In addition, the assayshowed to be quite specific for bisphenol A as well. Only for bisphenol A analogues, cross reactivitiesof about 40% were reached, enabling the use of the antibodies for the screening of bisphenol A andalike compounds. Keywords: Bisphenol A; immunoassay; IgY; ELISA; migration; chicken immunoglobulins; water; milk;cross reactivity; xeno-estrogens
|
