| Abstract
| - Immunoassays for 2,4,6-trichloroanisol (TCA) have been evaluated. The assays were developedafter raising antibodies against three different immunizing haptens (). Lack of reproducibility hasbeen one of the main problems of these assays. Precision was worse on these assays, reachinglower limits of detection. The high lipophilicity of TCA and its, consequently, low water solubility havebeen found to be the major cause of this problem. A reliable microplate-based enzyme-linkedimmunosorbent assay (ELISA) has been set after consideration of the TCA physicochemical featuresand evaluation of important parameters affecting immunoassay performance. The immunoassay usesAs78 (developed against hapten B-KLH) and C9-OVA as the coating antigen. The selectivity is highalthough the brominated analogue 2,4,6-TBA is also recognized. In buffered media containing 7%ethanol, the resulting assay shows a good accuracy with an IC50 value of 0.53 μg L-1 and a limit ofdetection of 0.044 μg L-1. Red and white wine samples caused important interferences in theimmunoassay demonstrating the necessity of a cleanup procedure prior to the ELISA. Keywords: Trichloroanisole; musty odor; wine; cork; immunoassay; lipophilicity
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