| Abstract
| - Fourier transform infrared (FTIR) spectroscopy with microattenuated total reflectance (mATR) samplingaccessory and chemometrics (partial least squares and principal component regression) was usedfor the simultaneous determination of saccharides such as fructose, glucose, sucrose, and maltosein honey. Two calibration models were developed. The first model used a set of 42 standard mixturesof fructose, glucose, sucrose, and maltose prepared over the range of concentrations normally presentin honey, whereas the second model used a set of 45 honey samples from various floral and regionalsources. The developed models were validated with different data sets and verified by high-performance liquid chromatography (HPLC) measurements. The R2 values between the FTIR-mATRpredicted and HPLC results of the different sugars were between 0.971 and 0.993, demonstratingthe predictive ability and accuracy of the procedure. Keywords: FTIR; ATR; chemometrics; HPLC; rapid; sugars
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