| Abstract
| - Polybrominated diphenyl ethers (PBDEs) are a class of brominated flame retardants that areincreasingly an environmental concern. Several antibodies were developed for the polybrominateddiphenyl ether flame retardant BDE-47 (1), often found in the highest concentration in human milk,plasma, and adipose tissue. Four haptens with different bromine and linker substitution patterns weresynthesized and utilized to generate five polyclonal antibodies from goats and two polyclonal antibodiesfrom rabbits. Competition was assessed using four different coating antigens for all seven antibodies.The coating antigen showed marked effects on competition. When the same hapten was used forantibody and the coating antigen less competition was observed. The effect of BDE structure oncompetition was evaluated by using BDE-47 (1), BDE-99 (2), BDE-100 (3), BDE-153 (4), andBDE-183 (5). None of the compounds showed high competition with antibody I-KLH, presumablybecause steric hindrance prevented formation of an efficient binding site. As predicted from structuralconsiderations, BDE-47 (1) competed well with the remaining antibodies, whereas BDE-100 (3)competed well with only II-KLH. The remaining congeners (BDE-99 (2), BDE-153 (4), and BDE-183(5)) contain bromines that cannot be positioned in binding sites and thus cross-react poorly. Thecompetition study demonstrated that a bromine substitution on the congener could occupy a positionanalogous to the linker's position. Keywords: ELISA; immunoassay; analysis; brominated flame retardants; PBDEs; polybrominateddiphenyl ether
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