| Abstract
| - Thin-layer chromatography with a flame ionization detector (TLC-FID) was used for monitoring theproduction of structured phospholipids (ML type: L, long-chain fatty acids; M, medium-chain fattyacids) by enzyme-catalyzed acidolysis between soybean phosphatidylcholine (PC) and caprylic acid.It was found that the structured PC fractionated into two to three distinct bands on both plate thin-layer chromatography (TLC) and Chromarod TLC. These three bands represented PC of the LLtype, ML type, and MM type, respectively. The TLC-FID method was applied in the present study toexamine the influence of enzyme dosage, reaction temperature, solvent amount, reaction time, andsubstrate ratio (caprylic acid/PC, mol/mol) on formation of ML-type PC in a batch reactor withThermomyces lanuginosa lipase as the catalyst. The formation of ML-type PC was dependent on allparameters examined except for the substrate ratio. The ML-type PC content increased with increasingenzyme dosage, reaction temperature, solvent amount, and reaction time. The substrate ratio hadno significant effect on the formation of ML-type PC within the tested range (3−15 mol/mol). Theformation of MM-type PC was observed in some experiments, indicating that acyl migration is takingplace during reaction since the lipase is claimed to be 1,3-specific. The TLC-FID method offers asimple and cheap technique for elucidation of product and byproduct formation during enzyme-catalyzed reactions for production of phospholipids containing mixtures of long- and medium-chainfatty acids. Keywords: Thermomyces lanuginosa lipase; acidolysis; response surface methodology; structuredphospholipids; phosphatidylcholine; TLC−FID
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