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Title
| - Event-Specific Real-Time Detection and Quantification ofGenetically Modified Roundup Ready Soybean
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Abstract
| - The event-specific real-time detection and quantification of Roundup Ready soybean (RRS) usingan ABI PRISM 7700 sequence detection system with light upon extension (LUX) primer was developedin this study. The event-specific primers were designed, targeting the junction of the RRS 5‘ integrationsite and the endogenous gene lectin1. Then, a standard reference plasmid was constructed thatcarried both of the targeted sequences for quantitative analysis. The detection limit of the LUX real-time PCR system was 0.05 ng of 100% RRS genomic DNA, which was equal to 20.5 copies. Therange of quantification was from 0.1 to 100%. The sensitivity and range of quantification successfullymet the requirement of the labeling rules in the European Union and Taiwan. Keywords: Real-time PCR; light upon extension (LUX); genetically modified organisms (GMOs); RRS
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