| Abstract
| - A new sensitive method for the simultaneous determination of 12 trichothecenes (deoxynivalenol,nivalenol, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, fusarenon X, T-2 toxin, HT-2 toxin,neosolaniol, monoacetoxyscirpenol, diacetoxyscirpenol, T-2 triol, and T-2 tetraol) by liquid chromatography−electrospray ionization tandem mass spectrometry (LC−ESI−MS/MS) is presented. Thedevelopment of the method and investigations on the matrix influence on the MS signal are describedin particular. The matrix effect was thereby minimized by using an internal standard, a special mobilephase, and specific fragmentation parameters. The sample was extracted with acetonitrile/water (84:16, v/v), and the extract was cleaned up with a MycoSep 227 column. Quantification was based onthe internal standard de-epoxy-deoxynivalenol. Calibration curves were linear between 16 and 1600ng/g, and the limits of detection ranged from 0.18 to 5.0 ng/g. The developed method was appliedfor the determination of trichothecenes in 120 naturally contaminated wheat and oat samples. Keywords: Trichothecenes; LC−MS/MS; food; cereals; analysis; matrix effects
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