| Abstract
| - The metabolomic analysis of 11 Ilex species, I. argentina, I. brasiliensis, I. brevicuspis, I. dumosavar. dumosa, I. dumosa var. guaranina, I. integerrima, I. microdonta, I. paraguariensis var.paraguariensis, I. pseudobuxus, I. taubertiana, and I. theezans, was carried out by NMR spectroscopyand multivariate data analysis. The analysis using principal component analysis and classification ofthe 1H NMR spectra showed a clear discrimination of those samples based on the metabolites presentin the organic and aqueous fractions. The major metabolites that contribute to the discrimination arearbutin, caffeine, phenylpropanoids, and theobromine. Among those metabolites, arbutin, which hasnot been reported yet as a constituent of Ilex species, was found to be a biomarker for I. argentina,I. brasiliensis, I. brevicuspis, I. integerrima, I. microdonta, I. pseudobuxus, I. taubertiana, and I.theezans. This reliable method based on the determination of a large number of metabolites makesthe chemotaxonomical analysis of Ilex species possible. Keywords: Metabolomic analysis; Ilex species; arbutin; phenylpropanoids; NMR; principal componentanalysis; classification
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