Four new flavonol 7-O-glycosides1−4 and a new chalcone ester glycoside 5have been isolatedfrom the leaves of Bidens leucantha along with four knownchalcone ester glycosides 6−9.Their structures were elucidated using a combination ofspectroscopic techniques. The in vitroanti-HIV activity of all the compounds was tested as part of ourscreening of potential anti-AIDS agents from medicinal plants. Although none of the flavonolglycosides was active, amoderate inhibitory effect on viral replication was exerted by chalconeester glycoside 9.